4 And the third let what was in his vessel come out into the rivers and the fountains of water; and they became blood. 5 And the voice of the angel of the waters came to my ears, saying, True and upright is your judging, O Holy One, who is and was from all time: 6 For they made the blood of saints and prophets come out like a stream, and blood have you given them for drink; which is their right reward. 7 And a voice came from the altar, saying, Even so, O Lord God, Ruler of all, true and full of righteousness is your judging.

8 And the fourth let what was in his vessel come out on the sun; and power was given to it that men might be burned with fire. 9 And men were burned with great heat: and they said evil things against the name of the God who has authority over these punishments; and they were not turned from their evil ways to give him glory. 10 And the fifth let what was in his vessel come out on the high seat of the beast; and his kingdom was made dark; and they were biting their tongues for pain. 11 And they said evil things against the God of heaven because of their pain and their wounds; and they were not turned from their evil works.

12 And the sixth let what was in his vessel come out on the great river Euphrates; and it became dry, so that the way might be made ready for the kings from the east. 13 And I saw coming out of the mouth of the dragon, and out of the mouth of the beast, and out of the mouth of the false prophet, three unclean spirits, like frogs.

Matthew Henry's Commentary on Revelation 16:4-13

Commentary on Revelation 16:1-7

(Read Revelation 16:1-7)

We are to pray that the will of God may be done on earth as it is done in heaven. Here is a succession of terrible judgments of Providence; and there seems to be an allusion to several of the plagues of Egypt. The sins were alike, and so were the punishments. The vials refer to the seven trumpets, which represented the rise of antichrist; and the fall of the enemies of the church shall bear some resemblance to their rise. All things throughout their earth, their air, their sea, their rivers, their cities, all are condemned to ruin, all accursed for the wickedness of that people. No wonder that angels, who witness or execute the Divine vengeance on the obstinate haters of God, of Christ, and of holiness, praise his justice and truth; and adore his awful judgments, when he brings upon cruel persecutors the tortures they made his saints and prophets suffer.

Commentary on Revelation 16:8-11

(Read Revelation 16:8-11)

The heart of man is so desperately wicked, that the most severe miseries never will bring any to repent, without the special grace of God. Hell itself is filled with blasphemies; and those are ignorant of the history of human nature, of the Bible, and of their own hearts, who do not know that the more men suffer, and the more plainly they see the hand of God in their sufferings, the more furiously they often rage against him. Let sinners now seek repentance from Christ, and the grace of the Holy Spirit, or they will have the anguish and horror of an unhumbled, impenitent, and desperate heart; thus adding to their guilt and misery through all eternity. Darkness is opposed to wisdom and knowledge, and forebodes the confusion and folly of the idolaters and followers of the beast. It is opposed to pleasure and joy, and signifies anguish and vexation of spirit.

Commentary on Revelation 16:12-16

(Read Revelation 16:12-16)

This probably shows the destruction of the Turkish power, and of idolatry, and that a way will be made for the return of the Jews. Or, take it for Rome, as mystical Babylon, the name of Babylon being put for Rome, which was meant, but was not then to be directly named. When Rome is destroyed, her river and merchandise must suffer with her. And perhaps a way will be opened for the eastern nations to come into the church of Christ. The great dragon will collect all his forces, to make one desperate struggle before all be lost. God warns of this great trial, to engage his people to prepare for it. These will be times of great temptation; therefore Christ, by his apostle, calls on his professed servants to expect his sudden coming, and to watch that they might not be put to shame, as apostates or hypocrites. However Christians differ, as to their views of the times and seasons of events yet to be brought to pass, on this one point all are agreed, Jesus Christ, the Lord of glory, will suddenly come again to judge the world. To those living near to Christ, it is an object of joyful hope and expectation, and delay is not desired by them.